Go to the Senior Technician's office using the link below, then click on the Methods Manual to see the table of contents. Obtain the following two SOPs by clicking on each one. You may wish to print them out.
Check your understanding of the SimuLab procedures for the cleaning and storage of glassware by undertaking the following activity.
Now that you are wearing the appropriate PPE and have obtained the SimuLab procedures for the cleaning and storage of glassware, go to the Analytical Laboratory and ensure that all glassware is clean and appropriately stored.
You have ensured that all the glassware in the Analytical Laboratory is clean and appropriately stored, ready for when it is needed. You can now proceed to the next task, which is to clean the laboratory benches.
Step 3. Prepare to clean the laboratory benches
Use the link below to obtain the SOP: General Disinfection Procedures from the Methods Manual in the Senior Technician's office.
The SOP: General Disinfection Procedures contains information about the SimuLab procedures for cleaning laboratory benches. After reading the SOP, go to the Analytical Laboratory and clean the benches using the link below.
Step 4. Making up an ethanol solution
You have been unable to clean the benches because the ethanol solution wash bottles are empty. You now need to make up two wash bottles containing 80% v/v ethanol solution so that the laboratory has an adequate supply of cleaning solution.
A safety requirement before using any chemical for the first time is that the Material Safety Data Sheet (MSDS) for that chemical must be read. The MSDS will include information on the properties of the chemical, any health hazard information, first aid guidelines, precautions for use and also safe handling information. The chemical supplier must supply an MSDS with any chemical it sells.
If you would like more information about MSDSs, visit the National
Occupational Health and Safety Commission (NOHSC) website. Use the
following link and then choose Web Visit - NOHSC MSDs Source
and also Web Visit - NOHSC MSDS: what is adequate information?
from the list given.
Use the following link to obtain the MSDS: Ethanol from the MSDS Folder in the Senior Technician's office.
Use the following link to do an activity after looking at the MSDS: Ethanol.
You notice from the MSDS that the PPE required when handling ethanol includes 'respirator as required'. You are unsure as to what this means, but need to ensure that you understand all the required safety procedures before handling ethanol.
Go to the Laboratory Supervisor's office using the link below, click on the person's image and choose the Ethanol Question in the list.
You are now ready to begin making up the 80% v/v ethanol solution but you cannot find a procedure for making the solution. You decide to ask the Senior Technician how the solution should be made up.
Go to the Senior Technician's office using the link below, click on the person's image and choose the 80% v/v Ethanol Solution Question in the list.
If you would like some further information before you make up your ethanol solution, go to the Resources and Training Room using the link below and click onto the Personal Study Books to see the table of contents. Obtain the following two Personal Studies by clicking on each one.
With the information you have obtained from Personal Study: Concentration of Solutions, Personal Study: % v/v, w/v, w/w and the Senior Technician's answer to the 80% v/v Ethanol Solution Question you should now know how you will make up the 80% v/v ethanol solution.
Choose the reagents, glassware and measuring equipment that you will need to make up the 80% v/v solution by clicking on the link below.
Make up enough 80% v/v ethanol solution to fill two wash bottles by clicking on the link below.
Step 5. Label ethanol wash bottles
For information on the labelling of chemicals click on the link below.
You will need to ensure that the ethanol wash bottles are labelled in accordance with SimuLab procedures. Click on the link below to obtain the SimuLab SOP: Labelling of Solutions.
Before you refill the empty ethanol wash bottles you will need to ensure that they are properly labelled. Click on the link below to check whether the empty ethanol wash bottles are appropriately labelled.
The ethanol wash bottles are not properly labelled. Click on the link below to label the wash bottles properly.
Now that the wash bottles are labelled you can fill them with the 80% v/v ethanol solution ready for use.
You can now clean the benches in the Analytical Laboratory.