You have now completed the knowledge content and activities for PMLTEST305A
Perform Aseptic Techniques. You may undertake the assessment if you are
ready or review the material in this unit again.
To be recognised as competent in this unit you must be assessed in both
the knowledge content and the workplace skills. The assessment is in two
parts, part one is a scenario that asks you to apply the knowledge you
have acquired in the unit and part two is a practical assessment.
The exercises in the following activity are designed to test your understanding
of the previous three sections. They are based on the information contained
in this unit of competency.
Test your understanding of aseptic techniques by answering the following
questions. Send the completed assessment to your tutor.
The following scenario provides an example of the range of laboratory
work which laboratory personnel may do to apply skills and knowledge covered
by the unit of competence 'PMLTEST305A Perform Aseptic Techniques'. Use
it to help you answer the assessment questions.
Sally is employed as a laboratory assistant in a large industrial
laboratory involved in the food industry. The laboratory carries out
a number of different types of tests and Sally is involved with many
of these. She works under the supervision of the food scientists and
plays a very important role in keeping the laboratory functioning.
The laboratory is responsible for testing the raw materials that are
used by the factory in the manufacture of products, which include
cheeses and yogurts. It also routinely tests the food products from
the 'line' at various times of the day.
Sally is involved with all of these tasks and often sees the tests
through from start to finish. It is important that Sally follows standard
procedures and protocol when carrying out her duties. The SOPs detail
the procedures and if Sally has any problems she can refer these to
the food scientist.
Some of the duties that Sally carries out involve:
preparing and staining specimens for microscopic examination
preparing cultures of microorganisms
using aseptic technique.
It is a busy laboratory and often Sally is asked to help in ensuring
there is no bacterial contamination of the products. This means that
she must know basic bacteriological techniques, such as how to spread
an agar plate.
Question 3 Why is it important to keep your laboratory clean? Briefly describe
the daily cleaning procedure in Sally's laboratory by:
sketching a plan of the laboratory and writing in the activities that
you perform in different parts of the work area
listing the main types of cleaning activities that are carried out
in each area on a daily basis (for example, disinfection of the benches,
sweeping floors, scrubbing sinks)
describing the areas that you need to clean, when they must be cleaned
and the cleaning agents or solutions that are used to clean these areas.
Sally is asked to subculture some bacterial cultures from one agar plate
to another. Describe the best method for streaking of agar plates to maximise
the potential for single colony growth and to avoid contamination.
Question 6 Sally has spilt a small amount of a bacterial broth culture on the
laboratory bench. What should she do to clean it up? Why is it important
to clean up chemical and microbial spills straight away?
Briefly explain your workplace procedure for cleaning up a small spill
of microbiological material containing a live culture.
Your answer should include the relevant safety precautions,
the equipment needed to clean up the spill and how the waste material
is collected for disposal.
After completing her tasks for today Sally would dispose of used items
correctly. Select the correct container based on your knowledge of the
SOP: Microbiological Waste Disposal for each of the different