You have now completed the knowledge content and activities for PMLTEST305A Perform Aseptic Techniques. You may undertake the assessment if you are ready or review the material in this unit again.

To be recognised as competent in this unit you must be assessed in both the knowledge content and the workplace skills. The assessment is in two parts, part one is a scenario that asks you to apply the knowledge you have acquired in the unit and part two is a practical assessment.

Part 1. Knowledge Assessment

The exercises in the following activity are designed to test your understanding of the previous three sections. They are based on the information contained in this unit of competency.

Test your understanding of aseptic techniques by answering the following questions. Send the completed assessment to your tutor.

The following scenario provides an example of the range of laboratory work which laboratory personnel may do to apply skills and knowledge covered by the unit of competence 'PMLTEST305A Perform Aseptic Techniques'. Use it to help you answer the assessment questions.

Sally is employed as a laboratory assistant in a large industrial laboratory involved in the food industry. The laboratory carries out a number of different types of tests and Sally is involved with many of these. She works under the supervision of the food scientists and plays a very important role in keeping the laboratory functioning.

The laboratory is responsible for testing the raw materials that are used by the factory in the manufacture of products, which include cheeses and yogurts. It also routinely tests the food products from the 'line' at various times of the day.

Sally is involved with all of these tasks and often sees the tests through from start to finish. It is important that Sally follows standard procedures and protocol when carrying out her duties. The SOPs detail the procedures and if Sally has any problems she can refer these to the food scientist.

Some of the duties that Sally carries out involve:

  • preparing and staining specimens for microscopic examination
  • making media
  • preparing cultures of microorganisms
  • using aseptic technique.

It is a busy laboratory and often Sally is asked to help in ensuring there is no bacterial contamination of the products. This means that she must know basic bacteriological techniques, such as how to spread an agar plate.

Question 1

Question 2

Question 3
Why is it important to keep your laboratory clean? Briefly describe the daily cleaning procedure in Sally's laboratory by:

  • sketching a plan of the laboratory and writing in the activities that you perform in different parts of the work area
  • listing the main types of cleaning activities that are carried out in each area on a daily basis (for example, disinfection of the benches, sweeping floors, scrubbing sinks)
  • describing the areas that you need to clean, when they must be cleaned and the cleaning agents or solutions that are used to clean these areas.

Question 4

Question 5
Sally is asked to subculture some bacterial cultures from one agar plate to another. Describe the best method for streaking of agar plates to maximise the potential for single colony growth and to avoid contamination.

Question 6
Sally has spilt a small amount of a bacterial broth culture on the laboratory bench. What should she do to clean it up? Why is it important to clean up chemical and microbial spills straight away?

  • Briefly explain your workplace procedure for cleaning up a small spill of microbiological material containing a live culture.
  • Your answer should include the relevant safety precautions, the equipment needed to clean up the spill and how the waste material is collected for disposal.

Question 7

Question 8

After completing her tasks for today Sally would dispose of used items correctly. Select the correct container based on your knowledge of the SOP: Microbiological Waste Disposal for each of the different wastes.

Send your answers to your Tutor.

Part 2. Practical Assessment

Practical assessment requires you to demonstrate the competency on-the-job (or in a simulated laboratory).

The assessment will consist of:

  • a demonstration of the competency in an on-the-job situation or a simulation
  • oral questioning about such things as laboratory specific knowledge of processes, troubleshooting and
  • questioning related to specific safety or other factors that may be peculiar to the learner's work environment
  • any relevant workplace documents that support the assessment.

If you are ready to undertake the practical assessment send a message to your tutor using the mail facility.

Use the following link to obtain a checklist to be used by your assessor.

Checklist: PMLTEST305A Perform Aseptic Techniques


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