You have now completed the knowledge content and activities for PMLTEST501A
Perform Microbiological Tests. You may undertake the assessment if you
are ready or review the material in this unit again.
To be recognised as competent in this unit you must be assessed in both
the knowledge content and the workplace skills. The assessment is in two
parts, part one is a written assessment of the knowledge you have acquired
in the unit and part two is a practical assessment.
The exercises in the following activity are designed to test your understanding
of the previous five tasks. They are based on the information contained
in this unit of competency.
You will not receive any feedback when you answer questions online, in
the assessment section.
You need to communicate with your tutor about how you undertake this assessment.
Your tutor may ask you to answer all the multiple choice questions and
perform all the activities in this section and then send them a print-out
of this completed page, along with answers to the assignment questions.
Test your understanding of microbiological tests by reading the following
scenario and answering the questions. Send the finished assessment to
A patient's urine sample and test request form have been brought to the
laboratory for urgent testing. You prepare the work area and yourself
for carrying out the required tests on this sample.
Examine the following Request for Testing Form that has come with the
sample shown below. What errors or omissions can you identify? List these
errors and describe what action you would take.
Request for Testing Form - Microbiology
Patient Name: D.O.B: 5/04/1968 Address: 28 Smith St, Noname Town Sex: Collection Date: 6/07/2004
Tests Requested: Identification of causative agent. Clinical Notes: Pain on urination. Doctor: Dr Paul Sample Description:
Describe the steps in the Gram stain technique. List four possible results
of this technique.
Later you prepare a Gram stained slide of the sample and examine it under
the light microscope. View the slide below.
Use diagrams to show your understanding of the term 'streaking' an agar
plate. Why is this an important technique to master? What does it allow
a technician to do?
Now that you have isolated the organism from the sample, you can proceed
to identify it using biochemical tests. You might like to refer to the
Flow Chart for Bacterial Identification by clicking on the link below
Using the information provided in the Unit explain how you would carry
out a Catalase Test. List the reagents and equipment you will need and
outline the procedure in clear steps.
If you were asked to count the number of microorganisms in the sample
what techniques could you use if you didn't have a microscope, or other
automated machine to do the counting? Outline the techniques you might
After carrying out the appropriate biochemical tests you have identified
the organism in the sample. What action do you take once you have completed
the testing? Who do you tell? How do you record the information?
Describe a method used to test the antibiotic sensitivity of a particular
microorganism. Use diagrams to illustrate your answer.