Where Am I ? >  Unit Introduction > Step 6
Step 6. Calibration and Setting Up of Equipment

You now have your purified lizard DNA in a form ready for analysis but before performing this analysis you need to set up the agarose gel equipment and pour the gel according to standard methods. Staff working in the DNA laboratory transferring samples.

This DNA has been amplified using PCR and appropriate primers and was then subjected to restriction enzyme digestion to break the DNA into smaller pieces. Restriction enzymes always cleave DNA at specific sequence of bases in a predictable and reproducible manner. Thus they are very useful for looking for relationships between samples of DNA.

An example of a restriction enzyme is Hae III (obtained from the bacterium Haemophilus aegypticus) that cleaves DNA as follows (remember that DNA is comprised of two complementary DNA strands so to cleave the DNA both strands must be cut):

GGdown arrow.CC
CCup arrow.GG

The DNA fragments produced are then analysed using the technique of agarose gel electrophoresis. Details of electrophoretic separation of DNA is beyond the scope of this unit but students may find additional information on electrophoresis in the following Study Notes that are available in the Resources and Training Room.

The following two Study Notes will provide the most information on DNA Electrophoresis.

study notes icon Study Notes: Denaturing Gels - DNA and RNA

study notes icon Study Notes: Horizontal and Vertical Gel Systems


The following Study Notes provide general information on DNA Electrophoresis.

study notes icon Study Notes: What's This Thing Called 'Electrophoresis' ?

study notes icon Study Notes: The Electrophoretic System

study notes icon Study Notes: The Electrophoresis Gel

study notes icon Study Notes: Electrophoresis Buffers

study notes icon Study Notes: Buffer Systems

For this step read the following SOP on Agarose Gel Electrophoresis before doing an activity on calibration and setting up of equipment.

manual icon MTH 111 SOP: Agarose Gel Electrophoresis

assignment icon Assignment: Agarose Gel Electrophoresis

 


   Where Am I ? >  Unit Introduction > Step 6