Where Am I ? >  Unit Introduction > Step 7
Step 7. Running the Gel

Once samples are loaded onto the gel and the electrical current applied the DNA fragments move through the gel. The smaller fragments move faster towards the bottom of the gel, and the larger fragments move slower and are found near the top of the gel. Gel tank with loaded samples attached to the top power pack.

This is the basis of separation. Once the smaller fragments approach the end of the gel, the gel apparatus is turned off and the gel removed.

UV - Transilluminator with camera attachment. The gel is then placed on a UV Transilluminator and the ethidium bromide that is added to the sample buffer and has attached to the DNA fragments (intercalated) causes the fragments to glow with an orange colour under UV light.

The bands are visualised by this method and photographed to provide a permanent record. Photo taken of PCR gel using the transilluminator .

Click on the following link to see the results of the lizard DNA analysis and determine from the results and controls presented the information that will be sent to the customer in the following step.

form icon Forms: Lizard DNA Results

 


   Where Am I ? >  Unit Introduction > Step 7