This animation shows a sample containing 3 different constituents (shown as red, green and blue dots) going into and down a column. As it goes down the column, the sample separates into 3 different coloured bands, the 3 constituents, and these move out of the column at 3 different times into 3 test tubes. The sample is therefore separated and a trace is produced at the same time (absorbance versus time), producing a peak as each constituent is removed.

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