UV-Visible Spectroscopy – Principles and Analysis

Section Introduction

UV-visible absorption spectroscopy is one of the most widely used techniques in clinical and chemical laboratories. It is employed in qualitative analysis for the identification of chemicals, however its principal use is for the quantitative determination of various organic and inorganic species in solution. This aspect will be the focus of this section.

The essential theory and principles of UV-visible spectroscopy, as well as its applications and limitations for chemical analysis, will be investigated.

There are five tasks you will undertake in this section.

  1. UV-Vis Absorption
  2. UV-Vis Instrumentation
  3. Quantitative Analysis
  4. Virtual Spectrophotometer
  5. Report Results

Complete each task in order, following the steps and instructions.

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