PMLTEST507A Apply Chromatographic and Electrophoretic Techniques

Unit Introduction

SimuLab Laboratories Pty. Ltd. is a small laboratory and as a Laboratory Technician your responsibilities include the use of sophisticated chromatographic and electrophoretic equipment to perform analytical tests. In the following sections you will concentrate on the principles of operation and testing related to HPLC (high performance liquid chromatography), GC (gas chromatography) and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).

You will also gain exposure to other techniques, specifically PC (paper chromatography), TLC (thin layer chromatography), affinity chromatography, gel filtration chromatography and western blotting.

Chromatography and electrophoresis are used to separate complex mixtures into their components and this process is used to identify and sometimes measure the levels of specified components in the samples. Your responsibilities extend to proper preparation to perform the work and finally to communicating the results to the client.

Note that the word ‘chromatography’ is commonly used to describe the family of chromatographic techniques. You will find that chromatography and its adjective – chromatographic - will be used frequently in this unit of study.


Link: Final Assessment Link: Report and Communicate Results Link: HPLC (High Performance Liquid Chromatography) Link: GC (Gas Chromatography) Link: Electrophoresis Link: Separation Science, Chromatographic and Electrophoretic Concepts Link: Prepare for Analytical Procedures

Getting started
To start this unit, click on one of the above sections. It is suggested that you complete all sections before attempting the Final Assessment.

Biotechnology in Action
If you would like to learn more about biotechnology in the laboratory click on the link below to access the biotechnology unit. This unit provides a brief introduction to some concepts of biotechnology and shows the application of the material covered in the units through the use of a scenario.

Biotechnology in Action

Documentation used in this unit
All SOPs etc. used in this unit are designed for training purposes only and should never be used in the workplace.

Mail tutor
Notify your tutor that you are starting this unit. Your tutor will then advise you on which sections you must complete online. Information about using electronic mail is available in the student guide.

Recognition of Current Competency (RCC)
RCC allows you to gain credit for skills and knowledge that you already have. For more information, use the RCC Information link below. Use the RCC Form (a Word document) to apply for RCC for this unit.

RCC Information

RCC Form

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